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DNA barcoding (Folmer et al. 1994), is an efficient method to distinguish species by short specific DNA sequences from a common region of their genome, such as the cytochrome c oxidase subunit 1 (CO1) gene. The early primers of Folmer et al. (1994) were not as universal as desired (Elias et al. 2007). Platyhelminthes (flatworms), being the fourth most speciose animal phylum, is a taxonomic group where universal barcoding primers are not very effective (Vanhove, et al. 2013). Recently, Van Steenkiste et al. (2014) and Elbrecht and Leese (2017) developed primers for parasitic and free-living flatworms that show promise as molecular barcodes. We tested the primers developed by Van Steenkiste et al. (2014), Dice 1F, Dice 11R, and Dice 14R, on a diverse collection of trematodes, a group with a significant number of undescribed taxa. A total of 120 amplifications were performed on 69 trematode samples from 27 genera. Dice 1F/11R and Dice 1F/14R primer sets were tested alongside the JB3/JB5 primer set (Bowles et al. 1992; Derycke et al. 2005). Overall amplification efficacy was notably larger for the Dice 1F/11R primer set. However, the JB3/JB5 primer pair led to a higher percentage of successful sequences, as compared to either of the Dice primers.
Dr. Anindo Choudhury, Biology and Environmental Science
biology, DNA barcoding, molecular prospecting
Elkin, Logan and Butak, WIll, "DNA Barcoding for Molecular Prospecting of Platyhelminthes" (2022). Student Presentations. 72.