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Description
Schmidtea mediterranea, the freshwater planarian, possesses protonephridia, which are an organized network of branched tubules that function in waste filtration and water regulation. On a cellular and molecular level, these structures show considerable homology to the human kidney making planarians a useful model system for exploring human excretory biology and disease (Scimone, 2011 and Vu, 2015). Additionally, the planarian genome contains many flatworm-specific protonephridia genes essential for survival, making them potential drug targets for parasitic worm infections of humans. We set out to validate the expression of protonephridia genes identified by Drop-seq, a single-cell RNA sequencing technique that identified the transcriptomes for every cell type in S. mediterranea (Fincher et al., 2018). To accomplish this, we mapped gene expression using colorimetric and fluorescence in situ hybridization and characterized gene functionality via RNA interference screening (RNAi).
Advisor
Dr. Ryan King, Biology
Publication Date
2022
Keywords
biology, gene expression, RNA interference screening
Recommended Citation
Gaetano, Andrew, "Functional Analysis of Protonephridia Genes Identified by Drop-Seq" (2022). Student Presentations. 38.
https://digitalcommons.snc.edu/collaborative_presentations/38
Comments
Click here to view the original presentation on Knight Domains.